Posted by: shrikantmantri | May 21, 2010

Science: Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

Published Online May 20, 2010
Science

 

DOI: 10.1126/science.1190719

RESEARCH ARTICLES

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

Daniel G. Gibson,1 

John I. Glass,1 

Carole Lartigue,1 Vladimir N. Noskov,1 Ray-Yuan Chuang,1Mikkel A. Algire,1 Gwynedd A. Benders,2 Michael G. Montague,1 Li Ma,1 Monzia M. Moodie,1Chuck Merryman,1 Sanjay Vashee,1 Radha Krishnakumar,1 Nacyra Assad-Garcia,1Cynthia Andrews-Pfannkoch,1 Evgeniya A. Denisova,1 Lei Young,1 Zhi-Qing Qi,1Thomas H. Segall-Shapiro,1 Christopher H. Calvey,1 Prashanth P. Parmar,1 Clyde A. Hutchison, III,2Hamilton O. Smith,2 J. Craig Venter1,2,*

We report the design, synthesis, and assembly of the 1.08-Mbp Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a Mycoplasma capricolum recipient cell to create new Mycoplasma mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletionsand polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.

1 The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA.
2 The J. Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA.

* To whom correspondence should be addressed. E-mail: jcventer@jcvi.org


Received for publication 9 April 2010. Accepted for publication 13 May 2010.

Source:http://www.sciencemag.org/cgi/content/abstract/science.1190719

Posted via email from Sharing significant bytes —(Shrikant Mantri)

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