Posted by: shrikantmantri | May 20, 2010

The Plant Journal: Changes in properties of wheat leaf cuticle during interactions with Hessian fly

Changes in properties of wheat leaf cuticle during interactions with Hessian fly

The Plant Journal

Early View (Articles online in advance of print)

Published Online: 16 Apr 2010

Dylan K. Kosma 1,†,‡ , Jill A. Nemacheck 2,† , Matthew A. Jenks 1 and Christie E. Williams 2,3,*

  1 Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA ,   2 USDA-ARS Crop Production and Pest Control Research Unit, MWA, West Lafayette, IN 47907, USA , and   3 Department of Entomology, Purdue University, West Lafayette, IN 47907, USA
  *For correspondence (fax 765 494 5105; e-mail Christie.Williams@ars.usda.gov).

  These authors contributed equally to this paper.

  Present address: Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA.

ABSTRACT

Infestation of wheat by Hessian fly larvae causes a variety of physical and biochemical modifications of the host plant. Changes occur in cuticle permeability, lipid composition and gene transcript abundance, and these responses differ substantially between resistant and susceptible wheat lines. Staining assays revealed that susceptible plants exhibited a generalized increase in leaf sheath epidermal permeability during infestation; whereas, epidermal permeability was only minimally affected in resistant plants. Furthermore, temporal profiling using gas chromatographic methods revealed that changes in cuticle lipid (wax and cutin) composition correlated well with differing levels of epidermal permeability in susceptible and resistant plants. Temporal analysis of cuticle-associated gene mRNA levels, by quantitative real-time PCR, indicated a relationship between transcript abundance and changes in cuticle lipid profiles of resistant and susceptible plants. Results suggest that conserving cuticle integrity via induction of specific wax constituents and maintenance of cutin amounts, determined by the accumulation of cuticle-associated transcripts, could be important components of wheat resistance to Hessian fly larvae.

Source: http://www3.interscience.wiley.com/journal/123356201/abstract

Posted via email from Sharing significant bytes —(Shrikant Mantri)

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