Posted by: shrikantmantri | April 1, 2010

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes

Nature 464, 721-727 (1 April 2010) | 

doi:10.1038/nature08869

; Received 22 September 2008; Accepted 22 January 2010

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes

Beate Neumann1,12, Thomas Walter1,12, Jean-Karim Hériché5,13, Jutta Bulkescher1, Holger Erfle1,3,13, Christian Conrad1,3, Phill Rogers1,13, Ina Poser6, Michael Held1,13, Urban Liebel1,13, Cihan Cetin3, Frank Sieckmann8, Gregoire Pau9, Rolf Kabbe10, Annelie Wünsche2, Venkata Satagopam4, Michael H. A. Schmitz7, Catherine Chapuis3, Daniel W. Gerlich7, Reinhard Schneider4, Roland Eils10, Wolfgang Huber9, Jan-Michael Peters11, Anthony A. Hyman6, Richard Durbin5, Rainer Pepperkok3 & Jan Ellenberg2

  1. MitoCheck Project Group,
  2. Gene Expression and,
  3. Cell Biology/Biophysics Units, Structural and,
  4. Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany
  5. Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
  6. Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany
  7. Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland
  8. Leica Microsystems CMS GmbH, Am Friedensplatz 3, D-68165 Mannheim, Germany
  9. European Bioinformatics Institute, European Molecular Biology Laboratory, Cambridge CB10 1SD, UK
  10. Division of Theoretical Bioinformatics, German Cancer Research Center, Im Neuenheimer Feld 267, D-69120 Heidelberg, Germany
  11. Institute for Molecular Pathology, Dr Bohr Gasse 7, A-1030 Vienna, Austria
  12. These authors contributed equally to this work.
  13. Present addresses: MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany (J.-K.H.); BIOQUANT Centre University Heidelberg, INF 267, D-69120 Heidelberg, Germany (H.E.); 3-V Biosciences GmbH, Wagistrasse 27, 8952 Schlieren, Switzerland (P.R.); Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland (M.H.); Karlsruhe Institute of Technology KIT, Herrmann-von-Helmholtz Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany (U.L.).

Correspondence to: Jan Ellenberg2 Correspondence and requests for materials should be addressed to J.E. (Email: jan.ellenberg@embl.de).

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Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the ~21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community.

Source:http://www.nature.com/nature/journal/v464/n7289/full/nature08869.html

Posted via email from Sharing significant bytes —(Shrikant Mantri)

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