Posted by: shrikantmantri | January 26, 2010

BiFC-FRET-FLIM and BiFC-FRET-APB to visualize PPI in plant cell

via Plant Physiology on 1/15/10


Publication Date: 2010 Jan 13 PMID: 20071602
Authors: Kwaaitaal, M. – Keinath, N. F. – Pajonk, S. – Biskup, C. – Panstruga, R.
Journal: Plant Physiol

Various fluorophore-based microscopic methods, comprising Forster Resonance Energy Transfer (FRET) and Bimolecular Fluorescence Complementation (BiFC), are suitable to study pairwise interactions of proteins in living cells. The analysis of interactions between more than two protein partners using these methods remains, however, difficult. In this study, we report the successful application of combined BiFC-FRET-FLIM (Fluorescence Lifetime Imaging Microscopy) and BiFC-FRET-APB (Acceptor Photobleaching) measurements to visualize the formation of ternary soluble N-ethylmaleimide sensitive factor attachment receptor (SNARE) complexes in leaf epidermal cells. This method expands the repertoire of techniques to study protein-protein interactions in living plant cells by a procedure capable of visualizing simultaneously interactions between three fluorophore-tagged polypeptide partners.

post to: CiteULike

Posted via email from Sharing significant bytes —(Shrikant Mantri)

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