Posted by: shrikantmantri | December 26, 2009

Nature Paper: Dynamics of nucleosome remodelling by individual ACF complexes

The chromatin remodeller ACF acts as a dimeric motor to space nucleosomes

 p1016

Lisa R. Racki, Janet G. Yang, Nariman Naber, Peretz D. Partensky, Ashley Acevedo, Thomas J. Purcell, Roger Cooke, Yifan Cheng & Geeta J. Narlikar

doi:10.1038/nature08621

The ATP-dependent chromatin assembly factor (ACF) generates and maintains nucleosome spacing by constantly moving a nucleosome towards the longer flanking DNA faster than the shorter flanking DNA. But how the enzyme moves back and forth between both sides of a nucleosome to accomplish bidirectional movement is unknown. Nucleosome movement is now shown to depend cooperatively on two ACF molecules, indicating that ACF functions as a dimer of ATPases.

Dynamics of nucleosome remodelling by individual ACF complexes

 p1022

Timothy R. Blosser, Janet G. Yang, Michael D. Stone, Geeta J. Narlikar & Xiaowei Zhuang

doi:10.1038/nature08627

The ATP-dependent chromatin assembly factor (ACF) generates regularly spaced nucleosomes, but the mechanism by which ACF mobilizes nucleosomes remains poorly understood. Here, single-molecule FRET is used to monitor the remodelling of individual nucleosomes by ACF in real time; the study reveals previously unknown remodelling intermediates and dynamics, and indicates that ACF is a highly processive and bidirectional nucleosome translocase.

Posted via email from Sharing significant bytes —(Shrikant Mantri)

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