Posted by: shrikantmantri | October 23, 2009

Bind-n-Seq: high-throughput analysis of in vitro protein-DNA interactions

via Nucleic Acids Research on 10/22/09

Publication Date: 2009 Oct 20 PMID: 19843614
Authors: Zykovich, A. – Korf, I. – Segal, D. J.
Journal: Nucleic Acids Res

Transcription factor-DNA interactions are some of the most important processes in biology because they directly control hereditary information. The targets of most transcription factor are unknown. In this report, we introduce Bind-n-Seq, a new high-throughput method for analyzing protein-DNA interactions in vitro, with several advantages over current methods. The procedure has three steps (i) binding proteins to randomized oligonucleotide DNA targets, (ii) sequencing the bound oligonucleotide with massively parallel technology and (iii) finding motifs among the sequences. De novo binding motifs determined by this method for the DNA-binding domains of two well-characterized zinc-finger proteins were similar to those described previously. Furthermore, calculations of the relative affinity of the proteins for specific DNA sequences correlated significantly with previous studies (R(2 )= 0.9). These results present Bind-n-Seq as a highly rapid and parallel method for determining in vitro binding sites and relative affinities.

post to: CiteULike

Posted via email from Sharing significant bytes —(Shrikant Mantri)


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